Case Study One : Inoculation of 12 fruiting bags (First Time Activity)

Inoculation : Gray Oyster Mushroom

8 Fruiting Bags Left for farther observation (4 Bags contaminated).

•   5.29.16 = 2 bags
•   Substrate (pure corrugated boxes), Sterilized. 
•   Result = 1 contaminated, 1 still under observation.
•        1st bag, contaminated
•        2nd bag, observation date : 6.16.16 ( 18 days after )
•            No proof of contamination, but no mycelia activities visible .

   

•  5.30.16 = 4 bags
•  Substrate ( pure corrugated boxes + lime,sugar,darak ) no specific ratio, Sterilized.
•  Result = All bags still under observation. 6.16.16 (17 days after )
•     The last bag is obviously successfully and actively being colonized by GO mycelia.
•     The First 3 bags, no contamination observed but with little mycelia  activities.

 

•  6.01.16 = 4 bags
•  Substrate (sawdust +darak+lime+sugar) no specific ratio, sterilized.
•  Result : 3 bags contaminated, one left for farther observation. 6.16.16 (15days after)
•   Update :  The last bag left observed to be also contaminated later in the afternoon(same day)
•  FINAL : 0/4 Success rate !

•  6.02.16 = 2 1.5 bottles
• Substrate (sawdust +darak+lime+sugar) no specific ratio, sterilized.
• Result : 2 1.5L bottles still under observation after 2 weeks. 6.16.16.
•         No contamination seen but not much mycelia activities observed. 

Case Study One  :

Contamination rate : 33.3% (4/12)

Success rate : 1/12 so far 7 so far still under observation.

Lesson learned : contamination rate is too high ! A more hygienic practice should be observe.

Update Result as follows...

Contamination rate now stand at 5/12 = more than 41.66% !